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Data analysis of images

I have microscope images of spherical particles. What I need is diameter information and center to center distance of the nearest neighbor for all the particles.

I am using ImageJ (which is a free image processing software). I create a black and white image and split clustered particles with Paint. I enter the scale information and calculate the area with ImageJ. In Excel I convert the area data to diameter and create a histogram.

For nearest neighbor distance, I find maxima of the images (with ImageJ, after entering the scale) which corresponds the center of the particles. (For clustered particles I mark the center with white before.) I transfer the coordinate data to Excel. I have a Macro which calculates the nearest neighbor distance for each particle. Then I create the histogram.

I have 44 images for distance, 45 for size calculation. I will provide tools, however you are free to develop and use yours. Precision is very important. If you are familiar with image processing it is not a hard project.

I have 6 sets of images. What I need is:

For size:

1 - Size distribution data for each image,

2 - Black an white mask image,

3 - Size distribution data of each set (combined data).

For distance:

1 - Distance distribution data for each image,

2 - Distance distribution data for each set.

You can see the samples here:

[url removed, login to view]

[url removed, login to view]

Habilidades: Arte & Artesanato, Engenharia, Pós-Produção, Pré-Produção

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( 4 comentários ) Canada

ID do Projeto: #3065593

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